the process of transcription involves copying genetic information from one strand of DNA into RNA . The enzyme responsible, DNA-dependent RNA polymerase, catalyses polymerisation in only one direction: 5' → 3' . Because of this directional requirement, the DNA strand with 3' → 5' polarity serves as the template strand . Mastering these molecular directions is a high-priority strategy, as NCERT textbooks provide the definitive foundation for approximately 95% of NEET Biology questions .