every chromosome visible during cell division has a primary constriction called the centromere . On the sides of this centromere, small disc-shaped structures called kinetochores are present . These kinetochores serve as the specific sites of attachment of spindle fibres to the chromosomes, allowing them to be moved and aligned at the equator (metaphase plate) during metaphase . While the centromere holds two chromatids together, the actual connection to the spindle apparatus is mediated by the kinetochores . Mastering these precise anatomical definitions is a high-priority strategy, as NCERT textbooks provide the foundation for approximately 95% of NEET Biology questions .