Bivalent or Tetrad formation is a characteristic feature observed during :
Synaptonemal complex in the zygotene stage
Chiasmata in the diplotene stage
Synaptonemal complex in the pachytene stage
Chiasmata in the zygotene stage
during the zygotene stage of prophase I, chromosomes start pairing together (synapsis). This process is accompanied by the formation of a complex structure called the synaptonemal complex. The text states, 'The complex formed by a pair of synapsed homologous chromosomes is called a bivalent or a tetrad' . Although they are more clearly visible as tetrads in the subsequent pachytene stage, the formation of the bivalent/tetrad structure via the synaptonemal complex is the defining characteristic of the zygotene stage.
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