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NEET BIOLOGYEasy

Choose the correct pair from the following :

A

Polymerases - Break the DNA into fragments

B

Nucleases - Separate the two strands of DNA

C

Exonucleases - Make cuts at specific positions within DNA

D

Ligases - Join the two DNA molecules

Step-by-Step Solution

  • Ligases are enzymes that join DNA fragments together (e.g., joining an antibiotic resistance gene with a plasmid vector) , .
  • Polymerases extend primers using nucleotides to synthesize/polymerise DNA, they do not break it .
  • Nucleases are enzymes that degrade DNA; specifically, Exonucleases remove nucleotides from the ends of the DNA, whereas Endonucleases make cuts at specific positions within the DNA .
  • Separation of DNA strands is typically achieved by denaturation (heat) or enzymes like helicases, not nucleases. Therefore, only the pair describing Ligases is correct.
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