Chargaff's rule states that for a double stranded DNA, the ratios between Adenine and Thymine and Guanine and Cytosine are constant and equal to one. RNA is typically single-stranded and does not necessarily follow this rule of base equivalence. RNA does possess 5' phosphoryl and 3' hydroxyl ends, contains heterocyclic nitrogenous bases, and can exhibit complementary base pairing (e.g., in secondary structures like tRNA).