You are given a tissue with its potential for differentiation in an artificial culture. Which of the following pairs of hormones would you add to the medium to secure shoots as well as roots?
IAA and gibberellin
Auxin and cytokinin
Auxin and abscisic acid
Gibberellin & abscisic acid
, in plant tissue culture, the nutrient medium must contain carbon sources, inorganic salts, vitamins, and growth regulators like auxins and cytokinins. Auxins generally promote root initiation (rooting), while Cytokinins promote cell division and shoot differentiation (shooting). The balance between these two hormones controls the differentiation of the callus into roots and shoots.
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