Plasmid pBR322 has PstI restriction enzyme site within gene that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain
(1) It will not be able to confer ampicillin resistance to the host cell
(2) The transformed cells will have the ability to resist ampicillin as well as produce -galactoside
(3) It will lead to lysis of host cell
(4) It will be able to produce a novel protein with dual ability
pBR322 is a commonly used cloning vector. When the gene for -galactoside is inserted in the ampicillin resistance gene by using Pst I, the recombinant E.coli will lose ampicillin resistance due to insertional inactivation of the antibiotic resistance gene. The host (recombinant) cell will produce -galactoside which is not a novel protein nor does it have dual ability. The transformed cells cannot resist ampicillin as they have lost ampicillin resistance. A recombinant E. coli is produced and the host cell will not undergo lysis due to insertion of -galactoside gene.
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