Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
Denaturation, Annealing, Extension
Denaturation, Extension, Annealing
Extension, Denaturation, Annealing
Annealing, Denaturation, Extension
The first step in the polymerase chain reaction is denaturation during which strands of dsDNA separate. This is followed by annealing in which primers anneal to 3' end of template DNA strand. Annealing is followed by extension in which Taq polymerase adds nucleotides to 3'OH end of primers.
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