The 3'-5' phosphodiester linkages inside a polynucleotide chain serve to join:
One nucleoside with another nucleoside
One nucleotide with another nucleotide
One nitrogenous base with pentose sugar
One DNA strand with the other DNA strand
two nucleotides are linked through a 3'-5' phosphodiester linkage to form a dinucleotide. More nucleotides can be joined in such a manner to form a polynucleotide chain. A nitrogenous base is linked to the pentose sugar through an N-glycosidic linkage to form a nucleoside, while hydrogen bonds connect the two DNA strands.
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