One scientist cultured Cladophora in a suspension of Azotobacter and illuminated the culture by splitting light through a prism. He observed that bacteria accumulated mainly in the region of:
Violet and green light
Indigo and green light
Orange and yellow light
Blue and red light
T.W. Engelmann (1843–1909) performed an experiment where he split light into its spectral components using a prism and illuminated a green alga, Cladophora, placed in a suspension of aerobic bacteria (like Azotobacter). He observed that the bacteria accumulated mainly in the region of blue and red light of the split spectrum. This accumulation occurred because these are the regions of maximum oxygen evolution, corresponding to the absorption spectra of chlorophyll a and b .
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