Semiconservative replication of DNA was first demonstrated in:
Drosophila melanogaster
Escherichia coli
Streptococcus pneumoniae
Salmonella typhimurium
the semiconservative mode of DNA replication was first shown in Escherichia coli and subsequently in higher organisms. This was demonstrated by Matthew Meselson and Franklin Stahl in 1958 using heavy nitrogen () and CsCl density gradient centrifugation.
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