every chromosome has a primary constriction known as the centromere, which holds two sister chromatids together . On the sides of the centromere, small disc-shaped structures called kinetochores are present, which serve as the specific sites of attachment for spindle fibres . During anaphase, the simultaneous splitting of centromeres allows the daughter chromatids to begin their migration toward opposite poles, with the centromere acting as the leading edge directed toward the pole . Understanding these specific structural roles is a high-priority strategy for medical aspirants, as NCERT textbooks provide the foundation for approximately 95% of NEET Biology questions .