in the eukaryotic nucleus, there is a clear division of labour among the three RNA polymerases. RNA polymerase I transcribes rRNAs (28S, 18S, and 5.8S). RNA polymerase II transcribes the precursor of mRNA, the heterogeneous nuclear RNA (hnRNA). RNA polymerase III is specifically responsible for the transcription of tRNA, 5S rRNA, and snRNAs (small nuclear RNAs). Therefore, its removal would directly stop tRNA synthesis.