Q1. Which biotechnological tool is used to amplify specific DNA sequences in vitro?A. Gel electrophoresisB. Polymerase Chain Reaction (PCR)C. ELISAD. Southern blotting
Q2. Which law in India addresses issues of biopiracy and patenting?A. Indian Biodiversity ActB. Indian Patents BillC. Environment Protection ActD. Wildlife Protection Act
Q3. Which of the following statements is correct about restriction enzymes?A. Restriction enzymes degrade mRNA molecules.B. Restriction enzymes cut DNA at specific recognition sites.C. Restriction enzymes are used to join DNA fragments.D. Restriction enzymes synthesize proteins from DNA.
Q4. Which of the following statements is incorrect about PCR?A. PCR is used to amplify specific DNA sequences.B. PCR requires DNA primers for amplification.C. PCR synthesizes amino acids for protein production.D. PCR is used in molecular diagnostics.
Q5. What is the function of Bt toxin in genetically modified crops?A. Increase nitrogen fixationB. Provide resistance against insectsC. Enhance photosynthesisD. Improve seed germination
Q6. Which regulatory body in India oversees research and safety of genetically modified organisms?A. ICMRB. GEACC. CSIRD. ICAR
Q7. Which vector is commonly used in plant genetic engineering for gene transfer?A. RetrovirusB. Ti plasmid of AgrobacteriumC. Lambda phageD. Cosmid
Q8. Which of the following is correct about gene therapy?A. Gene therapy introduces harmful mutations intentionally.B. Gene therapy is used to replace defective genes with functional ones.C. Gene therapy is unrelated to genetic disorders.D. Gene therapy prevents protein synthesis in cells.
Q9. Which of the following is correct about RNA interference (RNAi)?A. RNAi silences specific gene expression using double-stranded RNA.B. RNAi enhances gene expression in all organisms.C. RNAi is used for synthesizing DNA molecules.D. RNAi prevents DNA replication in bacteria.
Q10. Which molecule is produced by transgenic sheep for treating emphysema?A. Human insulinB. Alpha-1-antitrypsinC. Human growth hormoneD. Interferon