the large bundle sheath cells in C4 plants (Kranz anatomy) are characterized by having a large number of chloroplasts . While the mesophyll cells perform the initial C4 fixation, the Calvin pathway (biosynthetic phase) takes place specifically in the bundle sheath cells . These cells are rich in RuBisCO and allow the Calvin cycle to operate efficiently by maintaining a high concentration of CO2 . Photorespiration is absent in C4 plants , making option 2 incorrect.