Nowadays, it is possible to detect the mutated gene causing cancer by allowing the radioactive probe to hybridise its complementary DNA in clone of cells, followed by its detection using autoradiography because:
mutated gene does not appear on a photographic film as the probe has no complementarity with it.
mutated gene does not appear on a photographic film as the probe has complementarity with it.
mutated gene partially appears on a photographic film.
mutated gene completely and clearly appears on a photographic film.
In molecular diagnosis, a single-stranded DNA or RNA is tagged with a radioactive molecule (probe) and allowed to hybridise to its complementary DNA in a clone of cells. If the gene is mutated, the probe will not have complementarity with it and will not bind. Consequently, the clone with the mutated gene will not appear on the photographic film during autoradiography .
Join thousands of students and practice with AI-generated mock tests.