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NEET BIOLOGYGeneralMedium

Question

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

A

Denaturation, Annealing, Extension

B

Denaturation, Extension, Annealing

C

Extension, Denaturation, Annealing

D

Annealing, Denaturation, Extension

Step-by-Step Solution

The first step in the polymerase chain reaction is denaturation during which strands of dsDNA separate. This is followed by annealing in which primers anneal to 3' end of template DNA strand. Annealing is followed by extension in which Taq polymerase adds nucleotides to 3'OH end of primers.

Exam Context & Concepts Covered

This question aligns with the NEET BIOLOGY syllabus, specifically targeting concepts from General. Mastering this topic is crucial for scoring well in the upcoming medical entrance examinations. Solving conceptually related problems will help you understand the nuances of these concepts and improve your problem-solving speed.

BIOLOGYfollowingcorrectsequencepolymerasereaction

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