For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very importan

Question

For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
(1) Western Blotting Technique
(2) Southern Blotting Technique
(3) ELISA Technique
(4) Hybridization Technique

Accepted Answer

ELISA can be used for early detection of an infection either by detecting the presence of pathogenic antigen or by detecting the antibodies synthesized against the pathogen. Southern blotting is used to detect a specific DNA sequence in the given sample and can be detected prior to antibody formation. In hybridization technique a ssDNA/ssRNA tagged with a radioactive molecule (probe) is allowed to hybridize its complementary DNA in a clone of cells followed by detection using autoradiography. It is used to find a mutated gene. Western blotting technique is used to detect a specific protein molecule among a mixture of proteins.

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