Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A. Insertion of recombinant DNA into the host cell B. Cutting of DNA at specific location by restriction enzyme C. Isolation of desired DNA fragment D. Amplification of gene of interest using PCR. Choose the correct answer from the options given below :
B, C, D, A
C, A, B, D
C, B, D, A
B, D, A, C
The correct sequence for Recombinant DNA technology is: 1. Cutting of DNA at specific locations by restriction enzymes (B), 2. Isolation of the desired DNA fragment (C), 3. Amplification of the gene of interest using PCR (D), 4. Insertion of recombinant DNA into the host cell (A). Therefore, the sequence is B, C, D, A.
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