Which of the following is not a desirable feature of a cloning vector?
Presence of origin of replication
Presence of a marker gene
Presence of single restriction enzyme site
Presence of two or more recognition sites
Option (4) is the correct answer. Cloning vectors are the carriers of the desired gene in the host cell. The features desirable in a cloning vector are: Presence of origin of replication, Presence of marker genes, Presence of very few, preferably single recognition site for the commonly used restriction enzymes.
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