NEET Biology: Biotechnology Principles and Processes — Practice Set 13

Q1. Which of the following is NOT an application of recombinant DNA technology?

A. Gene therapy
B. Production of insulin
C. Fermentation of alcohol
D. Development of genetically modified crops

Q2. Which of the following statements about the Ti plasmid is incorrect?

A. The Ti plasmid is used to transfer genes into plant cells.
B. The Ti plasmid is derived from Agrobacterium tumefaciens.
C. The Ti plasmid is used in animal genetic engineering.
D. A disarmed Ti plasmid is used as a vector in plant biotechnology.

Q3. Which of the following techniques is used to insert recombinant DNA into host bacterial cells?

A. Electroporation
B. Centrifugation
C. Precipitation
D. Filtration

Q4. Which feature of restriction enzymes makes them suitable for recombinant DNA technology?

A. They cut DNA randomly
B. They cut DNA at specific recognition sites
C. They degrade unwanted proteins
D. They join DNA fragments

Q5. Which of the following is NOT a characteristic of restriction enzymes?

A. They recognize specific palindromic sequences
B. They cut DNA at specific sites
C. They always produce blunt ends
D. They are derived from bacteria

Q6. Which of the following statements about DNA ligase is correct?

A. DNA ligase is used to join DNA fragments by forming phosphodiester bonds.
B. DNA ligase is responsible for cutting DNA at specific recognition sites.
C. DNA ligase is not required in recombinant DNA technology.
D. DNA ligase prevents DNA replication.

Q7. Which of the following statements about transformation is correct?

A. Transformation is the process of introducing foreign DNA into bacterial cells.
B. Transformation always requires a viral vector.
C. Transformation cannot occur naturally.
D. Transformation is not used in genetic engineering.

Q8. Which of the following statements about plasmids is correct?

A. Plasmids are extra-chromosomal, circular DNA molecules.
B. Plasmids cannot replicate independently inside a host cell.
C. Plasmids are always linear in structure.
D. Plasmids cannot be used as vectors in genetic engineering.

Q9. Which property of restriction enzymes makes them useful in genetic engineering?

A. They randomly cut DNA
B. They degrade unwanted genes
C. They recognize and cut specific DNA sequences
D. They always cut DNA at the same position

Q10. What is the role of a vector in recombinant DNA technology?

A. To cut DNA at specific sites
B. To replicate foreign DNA inside a host
C. To amplify DNA sequences in PCR
D. To degrade unwanted genes

Biology — Biotechnology Principles and Processes

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Which of the following is NOT an application of recombinant DNA technology?

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