NEET Biology: Biotechnology Principles and Processes — Practice Set 9

Q1. Which of the following statements about plasmids is TRUE?

A. They are linear DNA molecules
B. They replicate independently of the host chromosome
C. They do not contain any genes
D. They are only found in eukaryotic cells

Q2. Which of the following is a crucial step before inserting recombinant DNA into a host organism?

A. DNA sequencing
B. Isolation of the desired gene
C. Protein synthesis
D. Southern blotting

Q3. Which of the following statements about gel electrophoresis is incorrect?

A. Gel electrophoresis separates DNA fragments based on size.
B. Smaller DNA fragments move faster than larger ones.
C. DNA moves toward the negative electrode during electrophoresis.
D. Gel electrophoresis requires an electric field.

Q4. What is the purpose of gel electrophoresis in recombinant DNA technology?

A. To separate DNA fragments based on size
B. To amplify DNA sequences
C. To replicate plasmid DNA
D. To transform bacterial cells

Q5. Which of the following statements about Southern blotting is correct?

A. Southern blotting is used to detect specific DNA sequences.
B. Southern blotting is used to detect proteins.
C. Southern blotting does not require gel electrophoresis.
D. Southern blotting is not useful in forensic science.

Q6. Which of the following statements about restriction enzymes is incorrect?

A. Restriction enzymes recognize specific DNA sequences and cut them.
B. Restriction enzymes are naturally found in bacteria.
C. All restriction enzymes produce sticky ends.
D. Restriction enzymes are used in recombinant DNA technology.

Q7. Which of the following statements about gel electrophoresis is incorrect?

A. It separates DNA fragments based on size.
B. DNA fragments move toward the negative electrode.
C. Agarose gel is commonly used for DNA electrophoresis.
D. Smaller DNA fragments migrate faster than larger fragments.

Q8. What is the function of DNA ligase in recombinant DNA technology?

A. To cut DNA at specific sites
B. To unwind the DNA helix
C. To join DNA fragments
D. To degrade unwanted nucleotides

Q9. Which of the following statements about vectors in genetic engineering is incorrect?

A. Vectors help in transferring foreign DNA into host cells.
B. Vectors must contain an origin of replication.
C. Vectors cannot contain selectable markers.
D. Plasmids and bacteriophages are commonly used as vectors.

Q10. Which of the following statements about cloning vectors is incorrect?

A. Cloning vectors contain an origin of replication.
B. Cloning vectors must have selectable markers.
C. Cloning vectors cannot replicate inside a host cell.
D. Plasmids and bacteriophages are common cloning vectors.

Biology — Biotechnology Principles and Processes

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Which of the following statements about plasmids is TRUE?

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