NEET Biology: Biotechnology Principles and Processes — Practice Set 7

Q1. Which of the following statements about PCR is incorrect?

A. PCR is used to amplify DNA sequences.
B. PCR requires thermostable DNA polymerase.
C. PCR involves three main steps: denaturation, annealing, and extension.
D. PCR is used to cut DNA at specific sites.

Q2. Which of the following statements about restriction enzymes is incorrect?

A. Restriction enzymes recognize specific DNA sequences.
B. All restriction enzymes produce sticky ends.
C. Restriction enzymes are used in recombinant DNA technology.
D. Restriction enzymes are derived from bacteria.

Q3. Which of the following statements about cloning vectors is incorrect?

A. Cloning vectors contain an origin of replication.
B. Cloning vectors lack selectable markers.
C. Cloning vectors are used to carry foreign DNA.
D. Cloning vectors can replicate independently inside host cells.

Q4. Which of the following statements about selectable markers is incorrect?

A. Selectable markers help distinguish transformed cells.
B. Selectable markers include antibiotic resistance genes.
C. Selectable markers inhibit bacterial growth.
D. Selectable markers help identify recombinant plasmids.

Q5. Which of the following statements about recombinant DNA technology is correct?

A. It involves the random mixing of genes
B. It allows insertion of specific genes into a host
C. It uses only bacterial cells as hosts
D. It does not involve any enzymes

Q6. Which enzyme is used to join DNA fragments during recombinant DNA technology?

A. Restriction enzyme
B. DNA polymerase
C. DNA ligase
D. Helicase

Q7. Which of the following statements about DNA ligase is correct?

A. DNA ligase joins DNA fragments by forming phosphodiester bonds.
B. DNA ligase is used to cut DNA at specific sites.
C. DNA ligase prevents DNA replication.
D. DNA ligase is not involved in recombinant DNA technology.

Q8. Which step in recombinant DNA technology involves making multiple copies of a gene?

A. Isolation of DNA
B. Insertion of DNA into a vector
C. Gene amplification using PCR
D. Selection of recombinant cells

Q9. Which method uses gold or tungsten particles to introduce foreign DNA into plant cells?

A. Microinjection
B. Biolistics
C. Electroporation
D. Liposome-mediated transfer

Q10. Which of the following is a key advantage of PCR over traditional cloning methods?

A. PCR requires living cells
B. PCR can amplify DNA in hours
C. PCR introduces fewer mutations
D. PCR does not require primers

Biology — Biotechnology Principles and Processes

Set 7 of 20

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Which of the following statements about PCR is incorrect?

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