NEET Biology: Biotechnology Principles and Processes — Practice Set 4

Q1. Which of the following statements about cloning vectors is correct?

A. Cloning vectors must contain an origin of replication.
B. Cloning vectors cannot carry foreign DNA.
C. Cloning vectors prevent DNA replication.
D. Cloning vectors must lack selectable markers.

Q2. Which of the following statements about genetically modified organisms (GMOs) is incorrect?

A. GMOs are created using recombinant DNA technology.
B. GMOs can be used to produce pharmaceuticals.
C. GMOs have no applications in medicine.
D. GMOs can be used in agriculture to improve crop yield.

Q3. What is the purpose of the origin of replication (ori) in a cloning vector?

A. To provide antibiotic resistance
B. To initiate DNA replication
C. To cut the DNA at specific sites
D. To enable transformation of the host cell

Q4. Which of the following statements about restriction enzymes is correct?

A. They always produce blunt ends
B. They recognize palindromic sequences
C. They randomly cut DNA
D. They unwind the DNA helix

Q5. Which of the following statements about DNA ligase is incorrect?

A. DNA ligase joins DNA fragments by forming phosphodiester bonds.
B. DNA ligase is required for recombinant DNA technology.
C. DNA ligase acts as a restriction enzyme.
D. DNA ligase is involved in DNA replication and repair.

Q6. Which of the following is the most commonly used host for cloning in recombinant DNA technology?

A. Streptococcus
B. Escherichia coli
C. Bacillus subtilis
D. Saccharomyces cerevisiae

Q7. Which of the following statements about cloning vectors is correct?

A. Cloning vectors contain an origin of replication.
B. Cloning vectors do not contain selectable markers.
C. Cloning vectors cannot carry foreign DNA.
D. Cloning vectors cannot replicate inside a host cell.

Q8. Which of the following statements about recombinant DNA technology is correct?

A. Recombinant DNA technology allows foreign genes to be inserted into host cells.
B. Recombinant DNA cannot replicate inside a host organism.
C. Recombinant DNA technology does not require restriction enzymes.
D. Recombinant DNA is always destroyed by host cells.

Q9. Which of the following enzymes is used to cut DNA at specific recognition sites?

A. DNA polymerase
B. Ligase
C. Restriction endonuclease
D. Reverse transcriptase

Q10. Which of the following serves as a selectable marker in cloning vectors?

A. Enhancers
B. Promoters
C. Antibiotic resistance genes
D. Silencers

Biology — Biotechnology Principles and Processes

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Which of the following statements about cloning vectors is correct?

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