Q1. Which type of restriction enzyme cuts DNA within a specific recognition site?A. ExonucleaseB. EndonucleaseC. PolymeraseD. Ligase
Q2. Which of the following statements about restriction enzymes is incorrect?A. Restriction enzymes are derived from bacteria.B. Restriction enzymes are used to cut DNA at specific sequences.C. All restriction enzymes produce sticky ends.D. Restriction enzymes recognize palindromic sequences.
Q3. What is the significance of the recognition site in restriction enzymes?A. It is the site where DNA replication startsB. It allows restriction enzymes to cut DNA at specific sequencesC. It prevents DNA degradationD. It helps in unwinding the DNA strands
Q4. Which of the following steps in recombinant DNA technology comes first?A. Insertion of recombinant DNA into the hostB. Isolation of desired DNAC. Amplification using PCRD. Screening of recombinant cells
Q5. Which of the following is used to make host cells competent for transformation?A. Heating at 100°CB. Treatment with calcium chlorideC. Addition of DNA ligaseD. Use of restriction enzymes
Q6. Which of the following statements about restriction enzymes is FALSE?A. They recognize specific DNA sequencesB. They cut DNA at recognition sitesC. They always produce sticky endsD. They are derived from bacteria
Q7. Which of the following best describes the term "transformation" in genetic engineering?A. Uptake of foreign DNA by a bacterial cellB. Mutation of DNA sequencesC. Translation of mRNA into proteinD. Transcription of DNA into RNA
Q8. Which of the following statements about cloning vectors is correct?A. Cloning vectors contain an origin of replication.B. Cloning vectors cannot carry foreign DNA.C. Cloning vectors cannot replicate inside host cells.D. Cloning vectors must not contain selectable markers.
Q9. Which of the following statements about polymerase chain reaction (PCR) is incorrect?A. PCR amplifies DNA sequences exponentially.B. PCR requires thermostable DNA polymerase.C. PCR does not require primers.D. PCR involves cycles of denaturation, annealing, and extension.
Q10. Which of the following statements about Southern blotting is correct?A. Southern blotting is used to detect specific DNA sequences.B. Southern blotting is used to amplify DNA sequences.C. Southern blotting does not require gel electrophoresis.D. Southern blotting is used to study protein expression.