NEET Biology: Biotechnology Principles and Processes — Practice Set 14

Q1. What is the role of a vector in recombinant DNA technology?

A. To cut DNA at specific sites
B. To replicate the foreign DNA inside a host
C. To amplify DNA sequences in PCR
D. To degrade unwanted genes

Q2. Which of the following statements about genetically modified organisms (GMOs) is correct?

A. GMOs are created using recombinant DNA technology.
B. GMOs cannot be used in agriculture.
C. GMOs have no medical applications.
D. GMOs are always harmful to the environment.

Q3. Which of the following statements about Southern blotting is incorrect?

A. Southern blotting is used to detect specific DNA sequences.
B. Southern blotting does not require gel electrophoresis.
C. Southern blotting involves DNA hybridization.
D. Southern blotting is useful in genetic fingerprinting.

Q4. Which of the following statements about bioreactors is incorrect?

A. Bioreactors provide optimal conditions for microbial growth.
B. Bioreactors are used for large-scale production of bioproducts.
C. Bioreactors cannot maintain sterile conditions.
D. Bioreactors regulate pH, oxygen levels, and temperature.

Q5. Which of the following statements about Southern blotting is incorrect?

A. Southern blotting is used to detect specific DNA sequences.
B. Southern blotting involves gel electrophoresis.
C. Southern blotting does not involve DNA hybridization.
D. Southern blotting is widely used in genetic research.

Q6. Which of the following statements about recombinant DNA is correct?

A. It is formed by joining DNA from different sources
B. It occurs only naturally
C. It does not replicate inside a host
D. It is not used in medical applications

Q7. Which of the following components is essential for maintaining sterility in bioprocess engineering?

A. High temperature
B. Sterile conditions
C. Antibiotic resistance genes
D. Use of multiple restriction enzymes

Q8. Which of the following statements about DNA ligase is correct?

A. DNA ligase joins DNA fragments by forming phosphodiester bonds.
B. DNA ligase is used to cut DNA at specific sequences.
C. DNA ligase inhibits DNA replication.
D. DNA ligase degrades foreign DNA.

Q9. Which of the following is a common application of recombinant DNA technology?

A. Production of genetically modified crops
B. Formation of fossils
C. Separation of proteins by centrifugation
D. Electrolysis of water

Q10. Which of the following enzymes is used in recombinant DNA technology to synthesize complementary DNA (cDNA) from mRNA?

A. DNA ligase
B. RNA polymerase
C. Reverse transcriptase
D. Endonuclease

Biology — Biotechnology Principles and Processes

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