Q1. Which process is used to transfer recombinant DNA into bacterial cells?A. ElectrophoresisB. TransformationC. ReplicationD. Southern blotting
Q2. Which of the following techniques is used to separate DNA fragments after restriction digestion?A. Southern blottingB. Gel electrophoresisC. Western blottingD. PCR
Q3. What is the primary function of polymerase chain reaction (PCR)?A. To cut DNA at specific sitesB. To amplify DNA sequencesC. To insert DNA into host cellsD. To synthesize proteins
Q4. Which feature allows plasmids to be used as vectors in genetic engineering?A. They are single-strandedB. They integrate into the host genomeC. They replicate independentlyD. They always carry antibiotic resistance genes
Q5. Which enzyme is used to produce sticky ends in a DNA fragment?A. DNA ligaseB. Reverse transcriptaseC. Restriction endonucleaseD. Helicase
Q6. Which enzyme is responsible for cutting DNA at specific recognition sites?A. DNA ligaseB. Restriction endonucleaseC. DNA polymeraseD. Reverse transcriptase
Q7. Which of the following statements about recombinant DNA technology is correct?A. Recombinant DNA technology allows foreign genes to be inserted into host cells.B. Recombinant DNA technology does not require ligases.C. Recombinant DNA is always unstable and degrades quickly.D. Recombinant DNA technology cannot be used for medical applications.
Q8. Which of the following statements about selectable markers is correct?A. Selectable markers help in identifying transformed cells.B. Selectable markers are not required in genetic engineering.C. Selectable markers prevent DNA replication.D. Selectable markers function only in eukaryotic cells.
Q9. Which of the following statements about gel electrophoresis is correct?A. Smaller DNA fragments migrate faster than larger fragments.B. DNA fragments move toward the negative electrode.C. All DNA fragments move at the same speed.D. Agarose gel electrophoresis is used to separate proteins.
Q10. Which of the following serves as a molecular carrier for gene transfer in bacteria?A. RibosomeB. PlasmidC. HistonesD. mRNA