Q1. Which of the following is NOT a component required for polymerase chain reaction (PCR)?A. DNA templateB. PrimersC. Taq polymeraseD. Ligase
Q2. Which of the following is an example of a vector used in plant genetic engineering?A. Lambda phageB. pBR322C. Ti plasmidD. Taq polymerase
Q3. Which of the following is a major advantage of genetic engineering over traditional hybridization?A. It allows precise introduction of desired genesB. It results in random mixing of traitsC. It requires multiple generations to show resultsD. It only works for asexual organisms
Q4. Which of the following vectors is used for gene transfer in plants?A. pBR322B. Lambda phageC. Ti plasmidD. pUC19
Q5. Which enzyme is responsible for sealing nicks in the sugar-phosphate backbone of DNA?A. DNA polymeraseB. LigaseC. HelicaseD. Endonuclease
Q6. Which method is used for large-scale production of recombinant proteins?A. MicroinjectionB. Southern blottingC. BioreactorsD. Northern blotting
Q7. Which of the following statements about genetically modified organisms (GMOs) is incorrect?A. GMOs are created using recombinant DNA technology.B. GMOs can be used in agriculture for pest resistance.C. GMOs have no applications in medicine.D. GMOs can be used to produce recombinant proteins.
Q8. Which feature of Agrobacterium tumefaciens makes it useful in genetic engineering?A. It naturally infects animal cellsB. It carries a tumor-inducing plasmidC. It produces antibioticsD. It can amplify DNA in PCR
Q9. Which of the following statements about cloning vectors is incorrect?A. Cloning vectors contain an origin of replication.B. Cloning vectors must have selectable markers.C. Cloning vectors cannot replicate inside a host cell.D. Plasmids and bacteriophages are commonly used as cloning vectors.
Q10. Which of the following statements about selectable markers is correct?A. Selectable markers help distinguish transformed cells from non-transformed cells.B. Selectable markers prevent bacterial growth.C. Selectable markers are only found in eukaryotic cells.D. Selectable markers inhibit DNA replication.