Q1. Which of the following methods is used to insert foreign DNA into plant cells?A. ElectroporationB. MicroinjectionC. BiolisticsD. Conjugation
Q2. Which of the following statements about restriction enzymes is incorrect?A. They cut DNA at specific recognition sites.B. They always produce blunt ends.C. They are derived from bacteria.D. They recognize palindromic sequences in DNA.
Q3. Which of the following is used to extract and purify DNA fragments from an agarose gel?A. ElectroporationB. ElutionC. HybridizationD. Transformation
Q4. Which of the following is NOT a feature of a cloning vector?A. Origin of replicationB. Selectable markerC. Multiple cloning sitesD. Exons and introns
Q5. What is the purpose of heating the DNA sample to 94°C during PCR?A. To activate DNA ligaseB. To denature the DNA strandsC. To allow restriction enzymes to cut DNAD. To prevent contamination
Q6. Which term refers to the ability of a plasmid to replicate independently inside a host cell?A. TransformationB. TransductionC. Autonomous replicationD. Conjugation
Q7. Which of the following methods can be used to introduce DNA into animal cells?A. ElectroporationB. ConjugationC. TransformationD. Southern blotting
Q8. Which enzyme is used to join DNA fragments in recombinant DNA technology?A. Restriction enzymeB. DNA polymeraseC. DNA ligaseD. Helicase
Q9. Which of the following techniques is used to separate DNA fragments?A. PCRB. Gel electrophoresisC. Southern blottingD. Western blotting
Q10. Which of the following statements about transformation is correct?A. Transformation is the process of introducing foreign DNA into bacterial cells.B. Transformation can only occur naturally and cannot be induced.C. Transformation always requires viral vectors.D. Transformation prevents the replication of recombinant DNA.